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RESEARCH PROFILE

Professor Dr. Pimchai Chaiyen

School of Biomolecular Science & Engineering (BSE)
Tel. +66 (0) 33 01 4200
Email pimchai.chaiyen@vistec.ac.th

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Bioreporter and Smart Detection Tools

 

Research Overview

Bioreporters are biological molecules that can generate measurable signals in response to specific chemical, physical or biological factors. The detection principle of this technology relies on the ability of detectors to recognize factors requiring detection, signal generation and display. This technology has broad applications in the areas of biomedicine, food and environment.

Bioreporter And Smart Detection At VISTEC

Ability of specific and smart detection has a broad impact on quality of life and can save tremendous resources otherwise spent in non-specific measurement. Our research team at VISTEC has enzymes that can bind to specific molecules and generate detectable signals in the forms of light or electricity.

We have developed proprietary technology based on an enzyme bacterial luciferase isolated in Thailand. This can be used as a gene reporter for specific gene detection. We are developing genetic systems that can be used as in vivo cell monitoring tools. This technology can track diseases inside experimental animals in real time and significantly reduce cost in drug discovery process.

We have enzymes that can react with pesticides and toxic chemicals and generate light signals. The technology is currently being developed as detection tools for environmental applications. Some of our enzymes can also bind to sugar and phenolic compounds and generate electricity. These reactions can be used as detection tools in food and environment applications.

 

Selected Publications

  • Maenpuen S, Tinikul S, Chenprakhon P and Chaiyen P. Production of Valuable Phenolic Compounds from Lignin by Biocatalysis In Emerging Areas in Bioengineering 2017; Wiley-VCH Verlag GmbH & Co., in press.
  • Pimviriyakul, P., Thotsaporn, K., Sucharitakul, J., Chaiyen, P. Journal of Biological Chemistry 2017; 292 (12), pp. 4818-4832.
  • Thotsaporn K, Tinikul R, Maenpuen S, Phonbuppha J, Watthaisong P, Chenprakhon P, and Chaiyen P. Journal of Molecular Catalysis B: Enzymatic 2016; 134, 353–366.
  • Dhammaraj T, Pinthong C, Visitsatthawong S, Tongsook C, Surawatanawong P, Chaiyen P. ACS Chem Biol. 2016;11(10):2889-2896.
  • Tinikul, R., Chaiyen, P. Structure, mechanism, and mutation of bacterial luciferase Advances in Biochemical Engineering/Biotechnology 2016, 154, pp. 47-74.
  • Visitsatthawong S, Chenprakhon P, Chaiyen P, and Surawatanawong P. Journal of the American Chemical Society 2015; 137(29):9363-74.
  • Dhammaraj T, Phintha A, Pinthong C, Medhanavyn D, Tinikul R, Chenprakhon P, Sucharitakul J, Vardhanabhuti N, Jiarpinitnun C, and Chaiyen P. ACS Catalysis 2015; 5:4492–4502.
  • Maenpuen S, Amornwatcharapong W, Krasatong P, Sucharitakul J, Palfey BA, Yuthavong Y, Chitnumsub P, Leartsakulpanich U, Chaiyen P. Journal of Biological Chemistry 2015; 290(13):8656-65.
  • Wongnate T, Surawatanawong P, Visitsatthawong S, Sucharitakul J, Scrutton NS, Chaiyen P. Journal of the American Chemical Society 2014; 136 (1): pp 241–253.
  • Chaiyen P, Fraaije M, Mattevi A. Trends in Biochemical Sciences 2012; 37(9): 373-80.
 

Research Group Members:

Dr. Pimchai Chaiyen (Professor)
Dr. Thanyaporn Wongnate (Lecturer)
Dr. Juthamas Jaroensuk (Postdoctoral Research Fellow)
Dr. Panu Pimviriyakul (Postdoctoral Research Fellow)
Ms. Pattarawan Intasian
Ms. Vinutsada Pongsupasa
Mr. Kridsadakorn Prakinee
Ms. Supacha Buttranon
Ms. Pangrum Punthong
Ms. Kittiya Sakdaphetsiri
Mr. Nattanon Akeratchatapan
Ms. Nuttanun Kutrakul
Mr. Thana Thaweeskulchai
Ms. Jittima Phonbuppha
Mr. Pratchaya Watthaisong
 

Collaborators:

Assistant Prof. Dr. Ruchanok Tinikul
Mahidol University
Dr. Somchart Maenpuen
Burapha University
Dr. Ubolsree Lertsakulpanich
NSTDA
 

International Research Collaborator:

Prof. Yoshihiro Ohmiya
AIST, Tsukuba, Japan