Welcome to Vidyasirimedhi Institute of Science and Technology. Website from Thailand

Prof. Dr. Pimchai Chaiyen ศ. ดร.พิมพ์ใจ ใจเย็น

Dean of School
Tel.  +(66) 33-014-220
Email  pimchai.chaiyen@vistec.ac.th

Chaiyen Research group website


Biography :
Name : Prof. Pimchai Chaiyen, Ph.D.
Main Position: Professor and Head
Address : School of Biomolecular Science and Engineering, Vidyasirimedhi Inistitute of Science and Technology (VISTEC), 555 Moo 1 Payupnai, Wangchan Valley, Rayong, Thailand 21210. 
  E-mail : pimchai.chaiyen@vistec.ac.th
  Website : www.vistec.ac.th/academic/home.php?school=BSE
Adjunct Position: Professor
Address : Department of Biochemistry / Center for Excellence in Protein and Enzyme Technology 
  Faculty of Science, Mahidol University 272 Rama VI Road, Bangkok 10400, THAILAND 
  E-mail : pimchai.cha@mahidol.ac.th
  Website : science.mahidol.ac.th/Chaiyen_P
1992 B.Sc. (First Class Hons) Chemistry, Prince of Songkla University,Thailand
1997 Ph. D. Biological Chemistry, University of Michigan, Ann Arbor, U.S.A.
Research and Professional Experiences:
2021-present Associate Editor, ACS Catalysis
2018-present Visiting Professor, Institute for Integrated Cell-Material Sciences (iCeMS), Institute for Advanced Study, Kyoto University, Japan
2018-present Visiting Professor, Biomedical Research Institute, Advanced Industrial Science and Technology (AIST),Tsukuba, Japan
2017-present Adjunct Professor, Dept of Biochemistry, Faculty of Science, Mahidol University
2017-present Professor and Head, School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC)
2009-2017 Professor, Dept of Biochemistry, Faculty of Science, Mahidol University
2005 Associate Prof, Dept of Biochemistry, Faculty of Science, Mahidol University
2001 Assistant Prof, Dept of Biochemistry, Faculty of Science, Mahidol University
1997 Lecturer, Dept of Biochemistry, Faculty of Science, Mahidol University
1994 Teaching assistant in Enzyme Kinetics for graduate students, University of Michigan, Ann Arbor
1993-1996 Teaching assistant in Biochemistry Laboratory for undergraduate and graduate students, University of Michigan, Ann Arbor
Contribution to Thai Policy in Biotechnology
2020-present Member of subcommittee for strategic plan for Bio-, Circular, and Green Economy Policy, Thai Government
2018-2020 Member of Subcommittee for Bio-Economy section, Eastern Economic Corridor (EEC)
2015-present Executive board member, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)
2015-2017 Member of Subcommittee for New Economy under the committees of Thailand’s National Reform Steering Assembly
Administration and Leadership Positions:
2017-present Head, School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC)
2016 Co-founder, Enzmart Biotech Co.
2011-2015 Deputy Dean for Research, Faculty of Science, Mahidol University
2009-2011 Chair, Dept of Biochemistry, Faculty of Science, Mahidol University
2008-2009 Deputy Chair, Dept of Biochemistry, Faculty of Science, Mahidol University
2018-present Acting Director, Frontier Research Center, Vidyasirimedhi Institute of Science and Technology (VISTEC)
Awards and Distinctions :
1985 Recipient of Distinguished Student Award from Princess Sirindhorn
1985-1997 Recipient of Scholarship from Development and Promotion of Science and Technology Talent Project
1993 Recipient of Dr.Tap Nilaniti Outstanding Graduate Award
1995 Recipient of Chrisman Award from Department of Biological Chemistry, University of Michigan, Ann Arbor. (for outstanding Ph.D. candidate)
1998 Recipient of Murphy Award from Department of Biological Chemistry, University of Michigan, Ann Arbor. (for the outstanding publication series)
2000 Recipient of Federation of Asian and Oceanic Biochemistry and Molecular Biology (FAOBMB) travel fellowship
2003 Recipient of the L'oreal-Unesco Fellowship for Woman in Science in Thailand
2005 Young Scientist Award, Foundation for the Promotion of Science and Technology under the Patronage of H. M. the King, Thailand.
2008 Affiliate Fellow of TWAS (The Academy of Sciences for the Developing World)
2009 BMB Award from Section of Biochemistry and Molecular Biology, The Science Society of Thailand under the Patronage of His Majesty the King.
2009 Faculty of Science Outstanding Lecturer Award (First-Middle Level)
2010 Exemplary Lecturer Award from Mahidol University Faculty Senate
2010 Taguchi Prize for Outstanding Research Achievement in Biotechnology
2010 TRF-CHE-Scopus Researcher Award 2010
2011 Outstanding Alumni Award 2011 from Prince of Songkla University
2012 2012 Outstanding Researcher Award (Chemical Sciences and Pharmacy Section) from the National Research Council of Thailand (NRCT)
2013 Received the title "TRF Senior Research Scholar" and  Research Team Building Grant from The Thailand Research Fund
2014 Chair of the Organizing Committee, IUBMB 18th International Symposium on Flavins and Flavoproteins
2015 Speaker at TEDx Bangkok 2015
2015 Outstanding Scientist of Thailand 2015, Foundation for the Promotion of Science and Technology under the Patronage of H. M. the King, Thailand.
2015 Outstanding Alumni Award from Royal Thai Government Scholarship Alumni Association
2016 BioTalk Plenary Awardee from Biotechnology and Biochemical Engineering Society of Taiwan (BEST)
2016 The project “bacterial luciferase as a gene reporter) received a special award from Leave A Nest Co. to participate in the Tech Planter Final Grand Prix in Tokyo, Japan (Sep 17, 2016)
2016 Received the title "TRF Senior Research Scholar" and Research Team Building Grant from The Thailand Research Fund
2017 Received Innovation Award for “Protein Markers” from the National Research Council of Thailand (NRCT)
2017 Received Research Excellence Award for “Serine hydroxymethyltransferase as a malarial drug target”
2017 Received First Place in Final Pitch Session “Leaders in Innovation Fellowship” hosted by Royal Academy     of Engineering and Newton Fund, UK 
2017 L'oreal-Unesco Woman in Science Crystal Award for the most accomplished woman scientist in Thailand (Life Science)
2019 Distinguished Alumni Lecture, Department of Biological Chemistry, University of Michigan, Ann Arbor, USA
2020 Research Excellence Award for "Pyranose 2-oxidase as an efficient biocatalyst for sugar conversion" from the National Research Council of Thailand (NRCT)
2021 Appointed Associate Editor ACS Catalysis
Editorial Board Members :
  The Journal of Biological Chemistry (JBC) (from July 2012-present)
  Archives of Biochemistry and Biophysics (from November 2012-present)
  eLife (from June 2019-present)
Ad Hoc Journal Reviewer :
  ACS Catalysis
  Acta Crystallographica Section F
  Applied Environmental Microbiology
  Applied Microbiology and Biotechnology
  Advanced Synthesis & Catalysis
  Archives of Biochemistry and Biophysics
  Biochimica Biophysica Acta
  Bioresource Technology
  Biotechnology Advances
  Biotechnology & Bioengineering
  Biotechnology Journal
  Chemistry & Biology
  Chemical Science
  FEBS Journal
  FEMS Microbiology Letter
  Journal of Agricultural and Food Chemistry
  The Journal of Biological Chemistry (JBC)
  Journal of Chemical Education
  Journal of Molecular Catalysis B: Enzymatic
  Journal of the American Chemical Society
  Journal of Photochemistry and Photobiology B: Biology
  Microbial Cell Factories
  Nature Chemical Biology
  Trends in Biochemical Science
Reviewer of Funding Organizations :
  Austrian Science Fund
  National Science Foundation (NSF, USA.)
  The Thailand Research Fund (TRF)
  National Science and Technology Development Agency (NSTDA)
  Commission on Higher Education (CHE), Ministry of Education
  Mahidol University
Field of Interest :
  Enzyme catalysis; Enzyme mechanism; Enzyme engineering
Biocatalysis; Biorefinery; Bioenergy
Biosensor; Bioreporter
Metabolic Engineering; Synthetic Biology
Flavins and Flavoproteins; Aldolase; Redox Enzymes; pyridoxal-5-phosphate enzymes
Publications :
Journal Articles
1. Chaiyen P, Brissette P, Ballou DP, Massey V. Reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase with N-methyl-5-hydroxynicotinic acid: Studies on the mode of binding, and protonation status of the substrate. Biochemistry 1997;36(45):13856-64.
2. Chaiyen P, Ballou DP, Massey V. Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Proc Nat Acad Sci U S A 1997;94(14):7233-8.
3. Chaiyen P, Brissette P, Ballou DP, Massey V. Unusual mechanism of oxygen atom transfer and product rearrangement in the catalytic reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Biochemistry 1997;36(26):8060-70.
4. Chaiyen P, Brissette P, Ballou DP, Massey V. Thermodynamics and reduction kinetics properties of 2-methyl-3- hydroxypyridine-5-carboxylic acid oxygenase. Biochemistry 1997;36(9):2612-21.
5. Chaiyen P, Suadee C, Wilairat P. A novel two-protein component flavoprotein hydroxylase p-hydroxyphenylacetate hydroxylase from Acinetobacter baumanniiEur J Biochem 2001;268(21):5550-61.
6. Chaiyen P, Sucharitakul J, Svasti J, Entsch B, Massey V, Ballou DP. Use of 8-Substituted-FAD Analogues to Investigate the Hydroxylation Mechanism of the Flavoprotein 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase. Biochemistry 2004;43(13):3933-43.
7. Thotsaporn K, Sucharitakul J, Wongratana J, Suadee C, Chaiyen P. Cloning and expression of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii: Evidence of the divergence of enzymes in the class of two-protein component aromatic hydroxylases. Biochim Biophys Acta 2004;1680(1):60-6.
8. Hommalai G, Chaiyen P, Svasti J. Studies on the transglucosylation reactions of cassava and Thai rosewood b-glucosidases using 2-deoxy-2-fluoro-glycosyl-enzyme intermediates. Arch Biochem Biophys 2005;442(1):11-20.
9. Oonanant W, Sucharitakul J, Yuvaniyama J, Chaiyen P. Crystallization and preliminary X-ray crystallographic analysis of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase from Pseudomonas sp. MA-1. Acta Crystallogr Sect F Struct Biol Cryst Commun 2005;61(3):312-4.
10. Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. The reductase of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii requires p-hydroxyphenylacetate for effective catalysis. Biochemistry 2005;44(30):10434-42.
11. Kujawa M, Ebner H, Leitner C, Hallberg BM, Prongjit M, Sucharitakul J, Ludwig R, Rudsander U, Peterbauer C, Chaiyen P, Haltrich D, Divne C. Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase. J Biol Chem 2006;281(46):35104-15.
12. Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. Kinetic mechanisms of the oxygenase from a two-component enzyme, p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumanniiJ Biol Chem 2006;281(25):17044-53.
13. Alfieri A, Fersini F, Ruangchan N, Prongjit M, Chaiyen P, Mattevi A. Structure of the monooxygenase component of a two-component flavoprotein monooxygenase. Proce Natl Acad Scie U S A 2007;104(4):1177-82.
14. Suadee C, Nijvipakul S, Svasti J, Entsch B, Ballou DP, Chaiyen P. Luciferase from Vibrio campbellii is more thermostable and binds reduced FMN better than its homologues. J Biochem 2007;142(4):539-52.
15. Sucharitakul J, Phongsak T, Entsch B, Svasti J, Chaiyen P, Ballou DP. Kinetics of a two-component p-hydroxyphenylacetate hydroxylase explain how reduced flavin is transferred from the reductase to the oxygenase. Biochemistry 2007;46(29):8611-23.
16. Nijvipakul S, Wongratana J, Suadee C, Entsch B, Ballou DP, Chaiyen P. LuxG is a functioning flavin reductase for bacterial luminescence. J Bacteriol 2008;190(5):1531-8.
17. Sucharitakul J, Prongjit M, Haltrich D, Chaiyen P. Detection of a C4a-Hydroperoxyflavin Intermediate in the Reaction of a Flavoprotein Oxidase. Biochemistry 2008;47(33):8485–90.
18. Spadiut O, Pisanelli I, Maischberger T, Peterbauer C, Gorton L, Chaiyen P, Haltrich D. Engineering of pyyranose 2-oxidase: improvement for biofuel cell and food applications through semi-rational protein design. J Biotechnol 2009; 139(3):250-7.
19. Prongjit M, Sucharitakul J, Wongnate T, Haltrich D, Chaiyen P. Kinetic mechanism of pyranose 2-oxidase from Trametes multicolor. Biochemistry 2009;48(19):4170-80.
20. Chosrowjan H, Taniguchi S, Mataga N, Phongsak T, Sucharitakul J, Chaiyen P, Tanaka F. Ultrafast Solvation Dynamics of flavin mononucleotide in the reductase component of p-Hydroxyphenylacetate Hydroxylase. J Phys Chem B 2009;113(25):8439-42.
21. Baron R, Riley C, Chenprakhon P, Thotsaporn K, Winter RT, Alfieri A, Forneris F, van Berkel WJH, Chaiyen P, Fraaije MW, Mattevi A, McCammon JA. Multiple pathways guide oxygen diffusion into flavoenzyme active sites. Proc Natl Acad Sci U S A 2009;106(25):10603-8.
  [ Cited in "Research Highlights" Nature Chemistry, 2009]
22. Sopitthammakhun K, Maenpuen S, Yuthavong Y, Leartsakulpanich U, Chaiyen P. Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues. FEBS J 2009;276(15):4023-36.
23. Maenpuen S, Sopitthammakhun K, Yuthavong Y, Chaiyen P, Leartsakulpanich U. Characterization of Plasmodium falciparum serine hydroxymethyltransferase—A potential antimalarial target. Mol Biochem Parasitol 2009;168(1):63-73.
24. Chaiyen P. Flavoenzymes catalyzing oxidative aromatic ring-cleavage reactions. Arch Biochem Biophys. 2010;493(1):62-70. (Review)
25. Pitsawong W, Sucharitakul J, Prongjit M, Tan TC, Spadiut O, Haltrich D, Divne C, Chaiyen P. A conserved active-site threonine is important for both sugar and flavin oxidations of pyranose 2-oxidase. J Biol Chem 2010;285(13):9697-705.
26. Sucharitakul J, Wongnate T, Chaiyen P. Kinetic isotope effects on the noncovalent flavin mutant protein of pyranose 2-oxidase reveal insights into the flavin reduction mechanism. Biochemistry 2010;49(17):3753-65.
27. Chenprakhon P, Sucharitakul J, Panijpan B, Chaiyen P. Measuring binding affinity of protein. Ligand interaction using spectrophotometry: binding of neutral red to riboflavin-binding protein. J Chem Edu 2010;87(8):829-31.
28. Rujkorakarn R, Nunthaboot N, Tanaka F, Chaiyen P, Chosrowjan H, Taniguchi S, Mataga N. Time-resolved Stokes shift in proteins with continuum model: Slow dynamics in proteins. J Photochem Photobiol A Chem 2010;215(1):38-45.
29. Tan TC, Pitsawong W, WongnateT, Spadiut O, Haltrich D, Chaiyen P, Divne C. H-bonding and Positive Charge at the N(5)/O(4) Locus are Critical for Covalent Flavin Attachment in Trametes Pyranose 2-Oxidase. J Mol Biol 2010;402(3):578-94
30. Nijvipakul S, Ballou SP, Chaiyen P. Reduction Kinetics of a Flavin Oxidoreductase LuxG from Photobacterium leiognathi (TH1):Half Sites Reactivity. Biochemistry 2010;49 (43):9241–8
31. Ruangchan N, Tongsook C, Sucharitakul J, Chaiyen P. pH-dependent studies reveal an efficient hydroxylation mechanism of the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. J  Biol Chem 2011;286(1):223-33.
32. Sucharitakul J, Wongnate T, Chaiyen P. Hydrogen peroxide elimination from C4A-hydroperoxy-flavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group. J Biol Chem 2011;286(19):16900-9.
33. Thotsaporn K, Chenprakhon P, Sucharitakul J, Mattevi A, Chaiyen P. Stabilization of C4A-hydroperoxy-flavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms. J Biol Chem 2011;286(32):28170-80.
34. Penpassakarn P, Chaiyen P, Palittapongarnpim P. Efficient heterologous expression and one-step purification of fully active c-terminal histidine-tagged uridine monophosphate kinase from Mycobacterium tuberculosis. Southeast Asian J Trop Med Public Health 2011;42(6):1452-1459.
35. Wongnate T, Sucharitakul J , and Chaiyen P. Identification of a catalytic base for sugar oxidation in pyranose 2-oxidase reaction. Chembiochem 2011;12(17):2577-86.
36. Tongsook C, Sucharitakul J, Thotsaporn K, Chaiyen P. Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. J Biol Chem 2011;286(52):44491–502.
37. Chenprakhon P, Panijpan B, Chaiyen P. An Experiment Illustrating the Change in Ligand pKa upon Protein Binding. J Chem Edu 2012; 89:791−795.
38. Chosrowjan H, Taniguchi S, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F. Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics. J Photochem Photobiol A Chem 2012;234(April 15):44-48.
39. Oonnanat W, Sucharitakul J, Chaiyen P, Yuaniyama J. Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii. Acinetobacter baumannii. Acta Crystallogr Sect F Struct Biol Cryst Commun 2012 Jun;68:720–723.
40. Sucharitakul J, Wongnate T, Montersino S, van Berkel, WJH, Chaiyen P. Reduction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1. Biochemistry 2012; 51(21):4309-21.
41. Somprasong N, Jittawuttipoka T, Duang-nkern J, Romsang, A, Chaiyen P, Schweizer H, Vattanaviboon P, Mongkolsuk S. Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation. J Bacteriol 2012;194(15):3904-12.
42. Sopitthummakhun K, Thongpanchang C, Vilaivan T, Yuthavong Y, Chaiyen P, Leartsakulpanich U. Plasmodium serine hydroxymethyltransferase as a potential anti-malarial target: inhibition studies using improved methods for enzyme production and assay, Malaria J 2012;11(1):194.
43. Phongsak T, Sucharitakul J, Thotsaporn K, Oonanant W, Yuvaniyama J, Svasti J, Ballou DP, Chaiyen P.The C-terminal domain of 4-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii is an auto-inhibitory domain. J Biol Chem 2012; 287(31):26213-26222.
44. Taniguchi S, Chosrowjan H, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F. Ultrafast Fluorescence Dynamics of Flavin Adenine Dinucleotide in Pyranose 2-Oxidases Variants and Their Complexes with Acetate: Conformational Heterogeneity with Different Dielectric Constants. J Photochem Photobiol A Chem 2012;245:33-42.
45. Chaiyen P, Fraaije M, Mattevi A. The Enigmatic Reaction of Flavins with Oxygen. Trends Biochem Sci 2012;37(9):373-80.
46. Tinikul R, Thotsaporn K, Taveekarn V, Jitrapakdee S, Chaiyen P. The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter. J Biotechnol 162 (2012):pp 346–353.
47. Tan TC, Spadiut O, Wongnate T, Sucharitakul J, Krondorfer I, Sygmund C, Haltrich D, Chaiyen P, Peterbauer CK, Divne C*. The 1.6 Å crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate Specificity and Reveals a Flavin Intermediate. PLoS One 2013;8(1):e53567.
48. Prongjit M, Sucharitakul J, Palfey BA, Chaiyen P*. Oxidation mode of pyranose 2-oxidase is controlled by pH. Biochemistry 2013 Feb 26;52(8):1437-45.
49. Wongnate T, Chaiyen P*. The substrate oxidation mechanism of pyranose 2-oxidase and other related enzymes in the glucose-methanol-choline superfamily. FEBS J 2013 Jul;280(13):3009-27. (Review)
50. Tinikul R, Pitsawong W, Sucharitakul J, Nijvipakul S, Ballou DP, Chaiyen P*. The transfer of reduced FMN from LuxG oxidoreductase to luciferase occurs via free diffusion. Biochemistry 2013 Oct 1;52(39):6834-6843.
51. Sucharitakul J*, Tongsook C, Pakotiprapha D, van Berkel WJ, Chaiyen P. The Reaction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodoccocus jostii RHA1 Provide an Understanding of the para-Hydroxylation Enzyme Catalytic Cycle. J Biol Chem. 2013 Dec 6;288:35210-35221.
52. Wongnate T, Surawatanawong P, Visitsatthawong S, Sucharitakul J, Scrutton NS, Chaiyen P. Proton-coupled Electron Transfer and Adduct Configuration are Important for C4a-Hydroperoxyflavin Formation and Stabilization in a Flavoenzyme. J Am Chem Soc. 2014;136(1):241–253.
53. Chitnumsub P, Ittarat P,  Jaruwat A,  Noytanom K,  Amornwatcharapong W, Pornthanakasem W,  Chaiyen P, Yuthavong Y, Leartsakulpanich U. Structure of Plasmodium falciparum serine hydroxymethyltransferase reveals a novel redox switch that regulates its activities. Acta Crystallogr D Biol Crystallogr. 2014 Jun;70(Pt 6):1517-27. 
54. Pinthong C, Maenpuen S, Amornwatcharapong W, Yuthavong Y, Leartsakulpanich U, Chaiyen P.Distinct Biochemical Properties of Human Serine Hydroxymethyltransferase Compared to the Plasmodium Enzyme: Implications for Selective Inhibition. FEBS J. 2014 Jun;281(11):2570-83.
55. Sucharitakul J, Tinikul R, Chaiyen P. Mechanisms of Reduced Flavin Transfer in the Two-component flavin-dependent Monooxygenases. Arch Biochem Biophys. 2014 Aug;555-556:33-46
56. Srisook T, Vongsetskula T*, Sucharitakul J, Chaiyen P, Tangboriboonrat P. Immobilization of 3-hydroxybenzoate 6-hydroxylase onto functionalized electrospun polycaprolactone ultrafine fibers: a novel heterogeneous catalyst. React Funct Polym 2014 Sep;82:41-46.  
57. Chenprakhon P , Trisrivirat D, Thotsaporn K , Sucharitakul J, Chaiyen P*. Control of C4a-hydroperoxyflavin protonation in the oxygenase component of p-hydroxyphenyl acetate-3-hydroxylase. Biochemistry 2014 Jul 1;53(25):4084-6. (Rapid Report)
58. Chitnumsub P, Jaruwat A, Riangrungroj P, Ittarat W, Noytanom K, Oonanant W, Vanichthanankul J, Chuankhayan P, Maenpuen S, Chen CJ, Chaiyen P, Yuthavong Y, Leartsakulpanich U. Structures of Plasmodium vivax serine hydroxymethyltransferase: Implications for ligand-binding specificity and functional control. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3177-86.
59. Sahin S, Wongnate T, Chaiyen P, Yu EH. Glucose oxidation using oxygen resistant pyranose-2-oxidase for biofuel cell applications. Chem Eng Trans 2014;41:367-372.
60. Messiha HL, Wongnate T, Chaiyen P, Jones AR, Scrutton NS. Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes. J R Soc Interface. 2015;12(103).
61. Panatdasirisuk, W., Vongsetskul, T., Sucharitakul, J., Chaiyen, P., Tangboriboonrat, P. Functionalized electrospun regenerated cellulose fibers for immobilizing pyranose 2-oxidase. React Funct Polym 2015;86:47-51.
62. Luanloet T, Sucharitakul J and Chaiyen P*. Selectivity of Substrate Binding and Ionization of 2-Methyl-3-Hydroxypyridine-5-Carboxylic Acid Oxygenase. FEBS J. 2015; 282(16):3107-25.
63. Elkhal C.K., Kean K, Parsonage D, Maenpuen S, Chaiyen P, Claiborne A and Karplus P.A. Structure and proposed mechanism of alpha-glycerophosphate oxidase from Mycoplasma pneumonia. FEBS J. 2015; 282(16):3030-42.
64. Maenpuen S, Amornwatcharapong W, Krasatong P, Sucharitakul J, Palfey BA, Yuthavong Y, Chitnumsub P, Leartsakulpanich U, Chaiyen P. Kinetic mechanism and the rate-limiting step of Plasmodium vivax serine hydroxymethyltransferase. J Biol Chem 2015;290(13):8656-65.
65. Maenpuen S, Watthaisong P, Supon P, Sucharitakul J, Parsonage D, Karplus PA, Claiborne A, Chaiyen P. Kinetic Mechanism of L-alpha-Glycerophosphate Oxidase from Mycoplasma pneumoniaeFEBS J. 2015; 282(16):3043-59.
66. Witschel MC, Rottmann M, Schwab A, Leartsakulpanich U, Chitnumsub P, Seet M, Tonazzi S, Schwertz G, Stelzer F, Mietzner T, McNamara C, Thater F, Freymond C, Jaruwat A, Pinthong C, Riangrungroj P, Oufir M, Hamburger M, Mäser P, Sanz-Alonso LM, Charman S, Wittlin S, Yuthavong Y, Chaiyen P, Diederich F. Inhibitors of plasmodial serine hydroxymethyltransferase (SHMT): Cocrystal structures of pyrazolopyrans with potent blood- and liver-stage activities. J Med Chem 2015;58(7):3117-30.
67. Dhammaraj T, Phintha A, Pinthong C, Medhanavyn D, Tinikul R, Chenprakhon P, Sucharitakul J, Vardhanabhuti N, Jiarpinitnun C, and Chaiyen P. p-Hydroxyphenylacetate 3-hydroxylase as a biocatalyst for the synthesis of trihydroxyphenolic acids.  ACS Catalysis 2015; 5:4492–4502.
68. Visitsatthawong S, Chenprakhon P, Chaiyen P, and Surawatanawong P. Mechanism of oxygen activation in a flavin-dependent monooxygenase: A nearly barrierless C4a-hydroperoxyflavin formation via Proton-coupled electron transfer. J Am Chem Soc 2015;137(29):9363-74.
69. Chaiyen P, Scrutton NS. Special Issue: Flavins and Flavoproteins: Introduction.
FEBS J. 2015 Aug;282(16):3001-2.
70. Graf M, Sucharitakul J, Bren U, Chu B, Koellensberger G; Hann S, Furtmüller P, Obinger C, Peterbauer C, Oostenbrink C. Chaiyen P, Haltrich, D. Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates. FEBS J. 2015 Nov;282(21):4218-41.
71. Sucharitakul J, Medhanavyn D, Pakotiprapha D, van Berkel W.  Chaiyen P. Tyr217 and His213 are Important for Substrate Binding and Hydroxylation of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii. FEBS J 2016 Mar;283(5):860-81.
72. Tinikul R and Chaiyen P. Structure, Mechanism, and Mutation of Bacterial Luciferase. Adv Biochem Eng Biotechnol. 2016;154:47-74.
73. Dhammaraj T, Pinthong C, Visitsatthawong S, Tongsook C, Surawatanawong P, Chaiyen P. A Single-site Mutation at Ser146 Expands the Reactivity of the Oxygenase Component of p-Hydroxyphenylacetate 3-Hydroxylase. ACS Chem Biol. 2016;11(10):2889-2896.
74. Thotsaporn K, Tinikul R, Maenpuen S, Phonbuppha J, Watthaisong P, Chenprakhon P, and Chaiyen P. Enzymes in the p-hydroxyphenylacetate degradation pathway of Acinetobacter baumannii. J Mol Catal B Enzym 2018;134:353–366.
75. Pruksatrakul T, PPhoopraintra P, Wilairat P, Chaiyen P, Chantiwas R. Development of a sequential injection-liquid microextraction procedure with GC-FID for analysis of short-chain fatty acids in palm oil mill effluent. Talanta 2017;165(1):612-618.
76. Pornsuwan S, Maenpuen S, Kamutira P, Watthaisong  P, Thotsaporn K, Tongsook C, Juttulapa M, Nijvipakul S, Chaiyen P. 3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Pseudomonas aeruginosa: An Fe(II)-containing enzyme with fast turnover. PLOS ONE 2017 Feb;12:e0171135.
77. Pimviriyakul P, Thotsaporn K, Sucharitakul J and Chaiyen P. Kinetic Mechanism of the Dechlorinating Flavin-Dependent Monooxygenase HadA. J Biol Chem. 2017 Mar 24;292(12):4818-4832
78. Chenprakhon P, Dhammaraj T, Chantiwas R, Chaiyen P. Hydroxylation of 4-hydroxyphenylethylamine derivatives by R263 variants of the oxygenase component of p-hydroxyphenylacetate-3-hydroxylase. Arch Biochem Biophys. 2017 Apr 15;620:1-11.
79. Schwertz G, Witschel MC, Rottmann M, Bonnert R, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat W, Schäfer A, Aponte RA, Charman SA, White KL, Kundu A, Sadhukhan S, Lloyd M, Freiberg GM, Srikumaran M, Siggel M, Zwyssig A, Chaiyen P, Diederich F. Antimalarial Inhibitors Targeting Serine Hydroxymethyltransferase (SHMT) with in Vivo Efficacy and Analysis of their Binding Mode Based on X-ray Cocrystal Structures. J Med Chem. 2017 Jun 22;60(12):4840-4860.
80. Amornwatcharapong W, Maenpuen S, Chitnumsub P, Leartsakulpanich P, and Chaiyen P. Human and Plasmodium Serine Hydroxymethyl Transferases Differ in Rate-limiting Steps and pH-Dependent Substrate Inhibition Behavior. Arch Biochem Biophys 2017;630:91-100.
81. Pinthong C , Phoopraintra P, Chantiwas R, Pongtharangkul T, Chenprakhon P, and Chaiyen P. Green and Sustainable Biocatalytic Production of 3,4,5-Trihydroxycinnamic Acid from Palm Oil Mill Effluent. Process Biochemistry. 2017;63:122-129
82. Schwertz G, Frei MS, Witschel MC, Rottmann M, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Ittarat A, Schäfer A, Aponte RA, Trapp N, Mark K, Chaiyen P, Diederich F. Conformational Aspects in the Design of Inhibitors for Serine Hydroxymethyltransferase (SHMT): Biphenyl, Aryl Sulfonamide, and Aryl Sulfone Motifs. Chemistry. 2017 Oct 12;23(57):14345-14357.
83. Sawasdee K, Sucharitakul J, Dhammaraj T, Niamsiri N, Chaiyen P, Prapainop K Encapsulation of the reductase component of p-Hydroxyphenylacetate Hydroxylase in poly (lactide-co-glycolide) nanoparticles by three different emulsification techniques. IET Nanobiotechnology. 2018 Jun;12(4):423-428. .
84. Tinikul R, Chenprakhon P, Maenpuen S, Chaiyen P. Biotransformation of Plant-Derived Phenolic Acids. Phenolic Acids. Biotechnol J. 2018 Jun;13(6):e1700632.
85. şahin S, Wongnate T, Chuaboon L, Chaiyen P, Yu EH. Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst. Biosens Bioelectron. 2018 Jun;107:17-25.
86. Teanphonkrang S, Janke S, Chaiyen P, Sucharitakul J, Suginta W, Khunkaewla P, Schuhmann W, Ruff A, Schulte A. Tuned Amperometric Detection of Reduced β-Nicotinamide Adenine Dinucleotide by Allosteric Modulation of the Reductase Component of the p-Hydroxyphenylacetate Hydroxylase Immobilized within a Redox Polymer. Anal Chem. 2018 May;90(9):5703–5711.
87. Schwertz G, Witschel MC, Rottmann M, Leartsakulpanich U, Chitnumsub P, Jaruwat A, Amornwatcharapong W, Ittarat W, Schäfer A, Aponte RA, Chaiyen P, and Diederich F. Potent Inhibitors for Plasmodial Serine Hydroxymethyltransferase (SHMT) Featuring a Spirocyclic Scaffold. ChemMedChem. 2018 May 8;13(9):931-943.
88. Phonbuppha J, Maenpuen S,  Munkajohnpong P, Chaiyen P, Tinikul R. A selective determination of the catalytic cysteine pKa of 2-cysteine succinic semialdehyde dehydrogenase from Acinetobacter baumannii using burst kinetics and enzyme adduct formation. FEBS J. 2018 Jul;285(13):2504-2519.
89. Yuenyaw A, Petchyam N, Kamonsutthipaijit N, Chaiyen P and Pakotiprapha D. Crystal structure of the flavin reductase of Acinetobacter baumannii p-hydroxyphenylacetate 3-hydroxylase (HPAH) and identification of amino acid residues underlying its regulation by aromatic ligands. Arch Biochem Biophys. 2018 Sep 1; 653:24-38.
90. Pimviriyakul P, Surawatanawong P, Chaiyen P. Oxidative Dehalogenation and Denitration by a Flavin-dependent Monooxygenase is Controlled by Substrate Deprotonation. Chemical Science. 2018 (9); 7468 – 7482.
91. Pimviriyakul P, Chaiyen P. A complete bioconversion cascade for dehalogenation and denitration by bacterial flavin-dependent enzymes. J Biol Chem. 2018 Nov 30;293(48):18525-18539.
92. Chenprakhon P, Wongnate T, Chaiyen P. Monooxygenation of aromatic compounds by flavin-dependent monooxygenases. Protein Sci. 2019 Jan;28(1):8-29.
93. Chuaboon L, Wongnate T, Punthong P, Kiattisewee C, Lawan N, Hsu CY, Lin CH, Bornscheuer U, Chaiyen P. One-Pot Bioconversion of L-Arabinose to L-Ribulose in an Enzymatic Cascade. Angew Chem Int Ed Engl. 2019 Feb 18;58(8):2428-2432.
94. Wongnate T, Surawatanawong P, Chuaboon L, Lawan N, Chaiyen P. The mechanism of sugar C-H bond oxidation by a flavoprotein oxidase occurs by a hydride transfer before proton abstraction. Chemistry. 2019 Mar 21;25(17):4460-4471.
95. Teanphonkrang S, Ernst A, Janke S, Chaiyen P, Sucharitakul J, Suginta W, Khunkaewla P, Schuhmann W, Schulte A, Ruff A. Amperometric detection of the urinary disease biomarker p-HPA by allosteric modulation of a redox polymer-embedded bacterial reductase. ACS Sens. 2019 May 24;4(5):1270-1278.
96. Duangpummet P, Chaiyen P, Chenprakhon P. Lipase-Catalyzed Esterification: An Inquiry-Based Laboratory Activity to Promote High School Students' Understanding and Positive Perceptions of Green Chemistry. J Chem Educ. 2019 May;96(6):1205-1211.
97. Ubonprasert S, Jaroensuk J, Pornthanakasem W, Kamonsutthipaijit N, Wongpituk P, Mee-Udorn P, Rungrotmongkol T, Ketchart O, Chitnumsub P, Leartsakulpanich U, Chaiyen P, Maenpuen S. A flap motif in human serine hydroxymethyl transferase is important for structural stabilization, ligand binding, and control of product release. J Biol Chem. 2019 Jul 5;294(27):10490-10502.
98. Jaroensuk J, Intasian P, Kiattisewee C, Munkajohnpon P, Chunthaboon P, Buttranon S, Trisrivirat D, Wongnate T, Maenpuen S, Tinikul R, Chaiyen P. Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway. J Biol Chem. 2019 Jul 26;294(30):11536-11548.
99. Pongpamorn P, Watthaisong P, Pimviriyakul P, Jaruwat A, Lawan N, Chitnumsub P, Chaiyen P. Identification of a Hotspot Residue for Improving the Thermostability of a Flavin-Dependent Monooxygenase. Chembiochem. 2019 Dec 13;20(24):3020-3031.
100. Watthaisong P, Pongpamorn P, Pimviriyakul P, Maenpuen S, Ohmiya Y, Chaiyen P. A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols. Angew Chem Int Ed Engl. 2019 Sep;58(38):13254-13258
- Highlighted as "Hot Paper:Biocatalysis" and "Frontispiece Article"
- Highlighted in more than 8 news outlets
101. Jaroensuk J, Wong YH, Zhong W, Liew CW, Maenpuen S, Sahili AE, Atichartpongkul S, Chionh YH, Nah Q, Thongdee N, McBee ME, Prestwich EG, DeMott MS, Chaiyen P, Mongkolsuk S, Dedon P, Lescar J, Fuangthong M. Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa. RNA. 2019 Nov;25(11):1481-1496.
102. Thongdee N, Jaroensuk J, Atichartpongkul S, Chittrakanwong J, Chooyoung K, Srimahaeak T, Chaiyen P, Vattanaviboon P, Mongkolsuk S, Fuangthong M. TrmB, a tRNA m7G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa. Nucleic Acids Res. 2019 Sep;47(17):9271-9281.
103. Songsungthong W, Yongkiettrakul S, Bohan LE, Nicholson ES, Prasopporn S, Chaiyen P, Leartsakulpanich U. Diaminoquinazoline MMV675968 from Pathogen Box inhibits Acinetobacter baumannii growth through targeting of dihydrofolate reductase. Sci Rep. 2019 Oct;9(1):15625.
104. Pimviriyakul P, Wongnate T, Tinikul R, Chaiyen P. Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions. Microb Biotechnol. 2020;13(1):67–86.
105. Jaroensuk J, Intasian P, Wattanasuepsin W, Akeratchatapan N, Kesornpun C, Kittipanukul N, Chaiyen P. Enzymatic reactions and pathway engineering for the production of renewable hydrocarbons. J Biotechnol. 2020 Feb 10;309:1-19.
106. Phonbuppha J, Tinikul R, Wongnate T, Intasian P, Hollmann F, Paul CE, Chaiyen P. A Minimized Chemoenzymatic Cascade for Bacterial Luciferase in Bioreporter Applications. Chembiochem 2020 Jul;21(14):2073-79.
107. Pitsawong W, Chenprakhon P, Dhammaraj T, Medhanavyn D, Sucharitakul J, Tongsook C, van Berkel WJH, Chaiyen P, Miller AF. Tuning of pKa values activates substrates in flavin-dependent aromatic hydroxylases. J Biol Chem. 2020 Mar 20;295(12):3965-3981.
108. Maenpuen S, Pongsupasa V, Pensook W, Anuwan P, Kraivisitkul N, Pinthong C, Phonbuppha J, Luanloet T, Wijma HJ, Fraaije MW, Lawan N, Chaiyen P, Wongnate T. Creating flavin reductase variants with thermostable and solvent-tolerant properties by rational-design engineering. ChemBioChem. 2020;21(10):1481-1491.
109. Munkajohnpong P, Kesornpun C, Buttranon S, Jaroensuk J, Weeranoppanant N, Chaiyen P. Fatty Alcohol Production: An Opportunity of Bioprocess. Biofuel Bioprod Bioref 2020;14:986-1009.
110. Woraruthai T, Kunno J, Pongsopon M, Yansakon K, Phoopraintra P, Chantiwas R, Leartsakulpanich U, Chaiyen P, Wongnate T. Chaiyen P. Identification and Cultivation of Hydrogenotrophic Methanogens from Palm Oil Mill Effluent for High Methane Production. Int J Energy Res 2020;44:10058-70.
111. Trisrivirat D, Lawan N, Chenprakhon P, Matsui D, Asano Y, Chaiyen P*. Mechanistic insights into the dual activities of the single active site of L-lysine oxidase/monooxygenase from Pseudomonas sp. AIU 813. J Biol Chem. 2020 Aug 7;295(32):11246-11261.
112. Pimviriyakul P, Chaiyen P. Overview of flavin-dependent enzymes. Enzymes. 2020;47:1-36.
113. Chenprakhon P, Pimviriyakul P, Tongsook C, Chaiyen P. Phenolic hydroxylases. Enzymes. 2020; 47:283-326.
114. Phintha A, Prakinee K, Chaiyen P. Structures, mechanisms and applications of flavin-dependent halogenases. Enzymes. 2020;47:327-364.
115. Pimviriyakul P, Chaiyen P. Flavin-dependent dehalogenases. Enzymes. 2020;47:365-397.
116. Chaiyen P, Tamanoi F. Preface: Flavin-Dependent Enzymes: Mechanisms, Structures and Applications. Enzymes. 2020;47:xv-xvi.
117. Patchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, Sappakhaw K, Leelahakorn N, Ruenkam T, Wongsatit T, Athipanyasilp N, Eiamthong B, Lakkanasirorat B, Phoodokmai T, Niljianskul N, Pakotiprapha D, Chanarat S, Homchan A, Tinikul R, Kamutira P, Phiwkaow K, Soithongcharoen S, Kantiwiriyawanitch C, Pongsupasa V, Trisrivirat D, Jaroensuk J, Wongnate T, Maenpuen S, Chaiyen P, Kamnerdnakta S, Swangsri J, Chuthapisith S, Sirivatanauksorn Y, Chaimayo C, Sutthent R, Kantakamalakul W, Joung J, Ladha A, Jin X, Gootenberg JS, Abudayyeh OO, Zhang F, Horthongkham N, Uttamapinant C. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020;4(12):1140-9.
118. Trisrivirat D, Hughes JMX, Hoeven R, Faulkner M, Toogood H, Chaiyen P, Scrutton NS. Promoter engineering for microbial bio-alkane gas production. Synth Biol (Oxf). 2020 Oct 27;5(1):ysaa022.
119. Phintha A, Prakinee K, Jaruwat A, Lawan N, Visitsatthawong S, Kantiwiriyawanitch C, Songsungthong W, Trisrivirat D, Chenprakhon P, Mulholland AJ, van Pee KH, Chitnumsub P, Chaiyen P. Dissecting the low catalytic capability of flavin-dependent halogenases. J Biol Chem. 2021 Jan;296:100068.
120. Sucharitakul J, Buckel W, Chaiyen P. Rapid kinetics reveal surprising flavin chemistry in the bifurcating electron transfer flavoprotein from Acidaminococcus fermentans. J Biol Chem. 2021;296:100124.
121. Kraithong T, Sucharitakul J, Buranachai C, Jeruzalmi D, Chaiyen P, Pakotiprapha D. Real-time investigation of the roles of ATP hydrolysis by UvrA and UvrB during DNA damage recognition in nucleotide excision repair. DNA Repair (Amst). 2021 Jan;97:103024.
122. Sucharitakul J, Buttranon S, Wongnate T, Chowdhury NP, Prongjit M, Buckel W, Chaiyen P. Modulations of the reduction potentials of flavin-based electron bifurcation complexes and semiquinone stabilities are key to control directional electron flow. FEBS J 2021 Feb;288(3):1008-26.
123. Pongpamorn P, Kiattisewee C, Kittipanukul N, Jaroensuk J, Trisrivirat D, Maenpuen S, and Chaiyen P. Carboxylic Acid Reductase Can Catalyze Ester Synthesis in Aqueous Environments. Angew Chem Int Ed Engl 2021 Mar;60(11):5749-53.
124. Tinikul R, Lawan N, Akeratchatapan N, Pimviriyakul P, Chinantuya W, Suadee C, Sucharitakul J, Chenprakhon P, Ballou DP, Entsch B, Chaiyen P. Protonation status and control mechanism of flavin–oxygen intermediates in the reaction of bacterial luciferase. FEBS J 2021:288(10):3246-3260.
125. Teanphonkrang S, Suginta W, Sucharitakul J, Fukamizo T, Chaiyen P, Schulte A. An electrochemical method for detecting the biomarker 4-HPA by allosteric activation of Acinetobacterbaumannii reductase C1 subunit. J Biol Chem. 2021 Jan-Jun;296:100467.
126. Nearmnala P, Thanaburakorn M, Panbangred W, Chaiyen P, Hongdilokkul N. An in vivo selection system with tightly regulated gene expression enables directed evolution of highly efficient enzymes. Sci Rep. 2021 Jun 3;11(1):11669.
127. Pimviriyakul P, Jaruwat A, Chitnumsub P*, Chaiyen P*. Structural insights into a flavin-dependent dehalogenase HadA explain catalysis and substrate inhibition via quadruple π-stacking. J Biol Chem. 2021 Aug;297(2):100952.
128. Intasian P, Prakinee K, Phintha A, Trisrivirat D, Weeranoppanant N, Wongnate T, Chaiyen P*. Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability. Chem Rev 2021;121(17):10367-10451.
129. Naramittanakul A, Buttranon S, Petchsuk A, Chaiyen P, Weeranoppanant N. Developments of a continuous-flow system with immobilized biocatalysts towards sustainable bioprocessing. React Chem Eng 2021;6(10):1771-1790.
130. Watthaisong P, Binlaeh A, Jaruwat A, Lawan N, Tantipisit J, Jaroensuk J, Chuaboon L, Phonbuppha J, Tinikul R, Chaiyen P, Chitnumsub P, Maenpuen S. Catalytic and structural insights into a stereospecific and thermostable Class II aldolase HpaI from Acinetobacter baumannii. J Biol Chem. 2021 Nov;291(5):101280.
131. Hemwech P, Obma A, Detsangiamsa, S, Wirasate S, Chaiyen P, Wilairat P, Chantiwas R. Capillary electrophoresis-UV analysis using silica-layer coated capillary for separation of seven phenolic acids and caffeine and its application to tea analysis. SN Appl Sci 2021;3:872.
132. Mekseriwattana W, Phungsom A, Sawasdee K, Wongwienkham P, Kuhakarn C, Chaiyen P, Katewongsa KP. Dual Functions of Riboflavin-functionalized Poly(lactic-co-glycolic acid) Nanoparticles for Enhanced Drug Delivery Efficiency and Photodynamic Therapy in Triple-negative Breast Cancer Cells. Photochem Photobiol, in press
133. Buttranon S, Jaroensuk J, Chaichol P, Chaiyen P, Weeranoppanant N. Reconfiguring workup steps in multi-cycle extractive bioconversion for sustainable fatty alcohol production: a process engineering approach. React Chem Eng, in press
Chapter in Book
Chaiyen P, Suadee C, Meevoothisom V. Degradation of phenylacetic acid by bacteria from Thai soil. In: Annual Report of ICBiotech. Vol. 21. Osaka: International Center for Biotechnology; 1998. p. 862-7.
Wongnate T and Chaiyen, P. Pyranose oxidases. In: Hille R., Miller, S.M., Palfey, B. (Eds.). Handbook of Flavoproteins. Oxidases, dehydrogenases and related systems. Volume 1. 2013. p. 177-193.
Tinikul R and Chaiyen P. Structure, Mechanism, and Mutation of Bacterial Luciferase In: Advances in Biochemical Engineering/Technology (Springer-Verlag Berlin Heidelberg) 2016; 154:47-74.
Maenpuen S, Tinikul S, Chenprakhon P and Chaiyen P.  Production of Valuable Phenolic Compounds from Lignin by Biocatalysis: State-of-the-Art Perspective. In Emerging Areas in Bioengineering (2018), Wiley-VCH Verlag GmbH & Co. pp 102-124
Patent :
2555 ยีนสำหรับฟิวชั่นลูซิเฟอเรสเอนไซม์จากเชื้อแบคทีเรีย Vibrio campbellii ที่เสถียรต่อภาวะอุณหภูมิสูงและองค์ประกอบของน้ำยาสำหรับวัดแอคติวิตี้เอนไซม์ลูซิเฟอเรส  สิทธิบัตร
2559 Fusion bacterial luciferase gene, reporter vector, and assay kit Patent Cooperation Treaty, PCT (PCT/TH/2016000051) 
2561 องค์ประกอบเครื่องหมายโปรตีน (Protein Marker) อนุสิทธิบัตร (เลขที่คำขอ 1503001543 เลขที่อนุสิทธิบัตร 14519) 
  องค์ประกอบเครื่องหมายโปรตีนที่ติดสี (Pre-stained Protein Marker) อนุสิทธิบัตร (เลขที่คำขอ 1503001542 เลขที่อนุสิทธิบัตร 13888) 
Papers in Proceedings
Chaiyen P, Suadee C, Thotsaporn K, Svasti J. Studies of the two-component p-hydroxyphenylacetate hydroxylase from Acinetobacter baumannii. In: Chapman S, Perham R, Scrutton N, editors. Flavins and Flavoproteins 2002: Proceedings of the Fourteenth International Symposium; 2002 July 14-18; St. John's College, University of Cambridge, Uk. Berlin: Agency for Scientific Publications; 2002. p. 975-80.
Sucharitakul J, Chaiyen P, Ballou DP, Massey V. Probing the mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase by using 8-substituted-FAD analogs. In: Chapman S, Perham R, Scrutton N, editors. Flavins and Flavoproteins 2002: Proceedings of the Fourteenth International Symposium; 2002 July 14-18; St. John's College, University of Cambridge, Uk. Berlin: Agency for Scientific Publications; 2002. p. 381-6.
Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. Mechanistic studies on the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii.. In: Nishino T, Miura R, Tanokura M, Fukui K, editors. Flavins and Flavoproteins 2005: Proceedings of the Fifteenth International Symposium; 2005 April 17-22; Shonan Village Center, Hayama, Japan: ARchiTect Inc.; 2005. p. 275-80.
Chaiyen P, Phongsak T, Sucharitakul J, Ballou DP. Review and perspectives of how reduced flavin is transferred between the reductase and oxygenase p-hydroxyphenylacetate 3-hydroxylase. In: Frago S, Gomez-moreno C, Medina M, editors. Flavins and Flavoproteins 2008: Proceedings of the Sixteenth International Symposium; 2008 June 8-13; Jaca, Spain: Prensas Universitarias de Zaragoza; 2008. p. 27-44.
Invited Lectures at International Symposiums:
Oral Presentation
“p-Hydroxyphenylacetic acid hydroxylase from Acinetobacter baumannii” 15th the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Symposium, Beijing, China, October 21-24, 2001.
Invited Seminar Lecture
"Reduced Flavin Transfer between Components of p-Hydroxyphenyl- acetate 3-Hydroxylase". A seminar at University of Pavia, Italy, June 12, 2006.
Invited Symposium Lecture
"How the reductase and oxygenase work together in a two-component flavoenzyme, p-hydroxyphenylacetate". 16th International Symposium on Flavins and Flavoproteins, Jaca, Spain , Jun 10, 2008.
4. Invited Speaker
Gordon Research Conference 2010: Enzymes, Co-enzymes & Metabolic Pathways "What is necessary for C4a-hydroperoxy-FAD formation in pyranose 2-oxidase reacion?" July 19-23, 2010 at Waterville Valley Resort, New Hampshire, USA.
5. Invited seminar lecture
" Exploring the Reaction Mechanism of Pyranose 2-Oxidase Through Transient Kinetics and Site-Directed Mutagenesis" at Wake Forest University, July 27, 2010, Winston-Salem, North Carolina, USA.
6. Invited Symposium lecture
“Oxygenation mechanism of p-hydroxyphenylacetate hydroxylase, a two-component flavin-dependent monooxygenase”. The 3rd Asia Pacific Protein Association (APPA) Conference, May 6-9, Shanghai, China.
7. Invited Symposium lecture
“Use of Kinetic Isotope Effects and Transient Kinetics to Unravel the Mechanism of H2O2 Elimination from C4a-Hydroperoxy-flavin in Pyranose2-Oxidase”. The IXth European Symposium of The Protein Society. May 22-26, 2011, Stockholm, Sweden.
8. Invited Symposium lecture
“Understanding reaction mechanisms of two-component flavin-dependent monooxygenases through a model of p-hydroxyphenylacetate hydroxylase”. 17th International Conference on Cytochrome P450, at The University of Manchester, UK 26th to 30th of June, 2011.
9. Oral Presentation
Mechanistic Studies of Flavin- and PLP-Dependent Enzymes. Presentation by TWAS young affiliate at 22nd General Meeting at International centre in Theroretical Physics, Trieste, Italy, on 21-23 November 2011.
10. Invited Speaker 
Gordon Research Conference 2012: Biocatalysis " Biocatalysis by Flavoenzymes:From Enzyme Mechanisms to Rational Engineering”
July 8-13, 2012, Bryant University, Smithfield, RI, USA.
11. Invited Symposium Lecture
"Control and Versatility in Catalysis by Flavin-dependent Enzymes"
Enzyme Engineering XXII: Emerging Topics in Enzyme Engineering
September 22-26, 2013, Toyama International Conference Center, Toyama, Japan 
12. Invited Lecture
"Bacterial Luciferase from Thailand as a Gene Reporter"
at BioMedical Research Institute, AIST, Tsukuba, Ibaraki, Japan
13. Invited Lecture
"Aromatic flavin-dependent monooxygenase as a regio-specific biocatalyst" 
May 12, 2014 at Institute of Biochemistry, University of Greifswald, Greifswald, Mecklenburg-Vorpommern, Germany.
14. Invited Lecture
“Oxygen Activation by Flavin-Dependent Enzymes” 
May 14, 2014 at The Max Planck Institute (MPI) for Terrestrial Microbiology, Marburg, Germany.
15. Invited Lecture
Invited Lecture “Mechanistic Studies of Flavin- and PLP-dependent Enzymes” 
at BASF-The Chemical Company, Ludwigshafen, Germany.
16. Invited Lecture
"Mechanisms of Oxygen Activation by Flavin-Dependent Enzymes" October 22, 2014 at 15th IUBMB - 24th FAOBMB-TSBMB International Conference, Academia Sinica, Taipei, Taiwan
17. Invited Lecture
"Mechanism and Biocatalysis of Flavin-Dependent Oxygenases" 13 November, 2014 at 9th Joint Conference on Chemistry Program Semarang, Indonesia.
18. Invited Lecture
Gordon Research Conference 2015: Enzymes, Co-enzymes & Metabolic Pathways "Oxygenation and Beyond by Two-component Flavin-dependent monooxygenases" July 12-17, 2015 at Waterville Valley Resort, New Hampshire, USA.
19. Invited Lecture
“Biotransformation of Aromatic Compounds by Flavin-Dependent Monooxygenases” July 26 – 30, 2015 at the 12th Biotrans, Vienna, Austria
20. Invited Lecture
"Two-component Flavin-Dependent Monooxygenases:
From Aromatic Hydroxylation to Light-Emitting Reaction" September 16, 2015
at BioMedical Research Institute, AIST, Tsukuba, Ibaraki, Japan
21. Invited Lecture
“From Mechanisms to Application of Two-component Flavin-Dependent Monooxygenases” Sep 19-20, 2015 at a regional meeting of Japan Society for Bioscience, Biotechnology and Agrochemistry, Toyama Prefectural University, Toyama, Japan
22. Invited Lecture
"Two-component Flavin-Dependent Monooxygenases: Challenge and Opportunity" Oct 9, 2015 at BallouFest Symposium 2015 in honor of Professor Dr. David P. Ballou, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.
23. Invited Lecture
"Bacterial Luciferase as a Eukaryotic Reporter System" at 19th International Symposium on Bioluminescence and Chemiluminescence, May 29 - June 2, Tsukuba, Japan.
24. Invited Lecture
(Plenary Lecture) "Oxygenation and Beyond by Two-Component Flavin-Dependent Monooxygenases" 21th BEST Conference June 24-25, 2016, National Central University, Taoyuan, Taiwan.
25. Invited Lecture
"From Mechanistic Understanding to Applications of Two-Component Flavin-Dependent Monooxygenases" at 8th European Meeting on OxiZymes, OxiZymes 2016, 3-6 July, 2016, The Netherlands.
26. Invited Lecture
(Plenary Lecture) " Flavin-Dependent Monooxygenases:One Intermediate for Many Reactions " The Fifth International Conference on Cofactors (ICC-05) and Active Enzyme Molecule 2016 (AEM 2016)” September 4 to 8, 2016,  Unazuki, Toyama, Japan. 
27. Invited Lecture
"Dehalogenase and Pyranose 2-oxidases and their applications" September 9, 2016 at R&D Headquarter, Amano Enzyme, Gifu, Japan.
28. Invited Lecture
“Flavin-Dependent Monooxygenases for Sustainable Technology” University of Bristol, Department of Chemistry, UK, March 2017
29. Invited Lecture
“Flavin-Dependent Monooxygenases for Sustainable Technology” University of Manchester, Manchester Institute of Biotechnology, UK, March 2017 
30. Invited Lecture
“Flavin-Dependent Monooxygenases for Sustainable Technology” Laboratory of Molecular Biology (LMB), Cambridge, UK, March 2017 
31. Invited Lecture
(Plenary Lecture) "Insights into Mechanisms of Flavin-Dependent Monooxygenases 
and Beyond " 19th International Symposium on Flavins and Flavoproteins, 
Groningen, The Netherlands, July 2-6, 2017
32. Invited Lecture
"Beyond Monooxygenation by Flavin-Dependent Enzymes " 20th International Conference on Cytochrome P450: Biochemistry, Biophysics and Biotechnology
 Düsseldorf, 27-31 August 2017
33. Invited Symposium Lecture
" From Fundamental to Novel Detection Technology of Flavin-Dependent Monooxygenases" 2018 Protein Science Society of Japan Annual Meeting, 26-28 June 2018, Niigata, Japan.
34. Invited Lecture
"From Fundamental Enzyme Catalysis To Applications in Detection and Green Chemistry" at Institute for Integrated Cell-Material Sciences (iCeMS), 29 June 2018, Kyoto, Japan
35. Invited Symposium Lecture
"Novel Enzymatic and Cascade Reactions for Biocatalysis and Biodetection"at EMBO workshop: Enzymes, biocatalysis and chemical biology: The new frontiers, 09 – 12 September 2018 Pavia, Italy, University of Pavia, Italy 9-12 September 2018
36. Invited Symposium Lecture
"Flavin-dependent dehalogease and halogenase"at 25 Year Biochemistry Workshopt at Technical University Dresden, Dresden, Germany, 28 September 2018
37. Invited Lecture
“Versatile Reactions of Two-Component Flavin-Dependent Monooxygenases” at Department of Experimental Biology & RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic, 1 November 2018
38. Invited Lecture
"Versatile Reactions of Two-Component Flavin-Dependent Monooxygenases" at Department of Biochemistry, Wageningen University, Wageningen, The Netherlands, 2 November 2018
39. Imvited Lecture
"Flavin-Dependent Enzymes: Mechanisms and Innovations" at Department of Chemistry, University of California, Berkeley, CA, USA 23 April 2019.
40. Distinguished Alumni Lecture
"Flavin-Dependent Enzymes: Mechanisms and Innovations" at Department of Biological Chemistry, University of Michigan, Ann Arbor, USA 25 April 2019.
41. Keynote Lecture
"Novel Enzymatic Cascades for Biodetection, Biocatalysis and Biofuel" at Southeast Asia Catalysis Conference (SACC), National University of Singapore, Singapore, 23-24 May, 2019
42. Invited Lecture
"Enzymatic Cascades for Biocatalysis, Biodetection and Biofuel" at Biotrans 2019, Groningen, The Netherlands. July 7-11, 2019.
43. Invited Lecture
27th FAOBMB & 44th MSBMB Conference and IUBMB Special Symposia, Berjaya Times Square Hotel, Kuala Lumpur, Malaysia. 19-22 August 2019.
44. Invited Lecture
"Microbial enzymatic systems for converting agro-industrial waste to useful compounds" at International Symposium on the Genetics of Industrial Microorganisms (GIM 2019), Pisa, Italy, 8-11 September 2019.
45. Invited Lecture
"Biodetoxification, Biosynthesis and Bioluminescence from Phenolic Toxicants: Three applications in a One-Pot Reaction" at An Annual Symposium of Japan Association for Bioluminescence and Chemiluminescence (JABC). Tsukuba, Japan. 5 October 2019.
46. Invited Lecture
at Multi-step Enzyme Catalyzed Processes Conference (MECP2020), Aachen, Germany. 30 March – 2 April, 2020.
47. Invited Lecture
at the World Conference on Protein Science, Sapporo, Japan. 7-10 July, 2020.
48. Invited Lecture
at Multistep Enzyme Catalyzed Processes Conference (MECP2020+1), Aachen, Germany. September 13-16, 2021.
49. Invited Lecture
at AFOB 2021 Virtual Conference, November 1-4, 2021.
50. Invited Lecture
at Pacifichem 2021, Hawaii (online conference), December 16-21, 2021.
Invited Symposium Lectures in Thailand
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See more at Chaiyen's group website